Last updated 2007.04.26
Real-Time PCR Technology Suitable for Detecting Waterborne Pathogens
May 14th - 18th, 2007 Tucson, AZ
Offered by: Arizona Research Laboratories, NSF - Water Quality Center - University of Arizona, National Center for Biotechnology Information, Applied Biosystems
Location: The University of Arizona, Koffler 541
Fee: $750 Government and Academic Labs, $1100 Commercial Labs
Registration: We have limited enrollment to maximize individual instruction and hands-on experience.
FOR FULL WORKSHOP REGISTRATION ONLY: REGISTER HERE to get listed and MAIL THE REGISTRATION FORM back with your payment.
FOR TUESDAY MAY 15th ONLY: REGISTER HERE for the
portion of the workshop. This portion is free of charge (1 day).
Accommodations: Attendees will need to provide their own transportation and accommodations. Choose from several hotels and motel in The University of Arizona area. Follow this link for accomodations in the Tucson area.
About the Course and Target Audience
This course is intended for laboratories interested in implementing or updating the use of real-time PCR for detecting bacterial, protozoan and viral pathogens in water samples. We are also focusing on the use of bioinformatics to introduce concepts and techniques for searching DNA sequences in the sequence database. The National Center for Biotechnology Information (NCBI) will be providing 1 full day of hands-on training DNA sequence searching using NCBI Tools (This portion of the workshop is free and open to all interested - attendees must REGISTER HERE).
- Municipal/County/State Water Utilities
- Waste Water Departments
- Academic Labs
- Non-Profit Water Testing Laboratories
- Commercial Laboratories
Course Outline
This is a custom workshop and is being updated regularly.
Seminars and Tours
Pathogens and Indicators in Water
Detection Systems in Research and in Current Practice
State-of-the-art Detection Systems
Plus Others
Background and Hands-on Workshop
Sample Purification Systems
Introduction of PCR Technology and Methodology
Theory: Real-Time PCR
SYBR Green and TaqMan Assays
State-of-the-art Detection Technologies
Advantages and Limitations of the Current Technologies
Bioinformatics and Computer Lab
Experiment Design
Primer Design Principles
Data Interpretation
Trouble-Shooting and Optimization Strategies
Plus Others
Important Note
Our teaching laboratories are not certified for handling and testing of pathogenic organisms. We will only use strains and samples that DO NOT contain pathogens. The purpose of the course is to simulate the testing of the organisms by using purified DNA samples or treated samples. Please do not bring samples containing live pathogens to the workshop.
Need Additional Info?
Contact Al Agellon agellon@email.arizona.edu
