Real-Time PCR Technology Suitable for Detecting Waterborne Pathogens

Workshop Series in Molecular Biology

This workshop has been rescheduled for Spring 2007.

(last updated 2006.09.13)

Offered by: ARLDB
Location: The University of Arizona
Fee: To be determined

Registration:

Accommodations:

About the Course and Target Audience

This course is intended for laboratories interested in implementing or updating the use of real-time PCR for detecting bacterial and viral pathogens in water samples.

Course Outline

This is a custom workshop - please inquire.

Background and Hands-on Workshop

Introduction of PCR Technology

Theory: Real-Time PCR

State-of-the-art detection technologies

Advantages and limitations of the current technologies

Bioinformatics

Experiment design

Primer design principles

Data interpretation

Trouble-shooting and optimization strategies

Important Note

Our teaching laboratories are not certified for handling and testing of pathogenic organisms. We will only use strains and samples that DO NOT contain pathogens. The purpose of the course is to simulate the testing of the organisms by using purified DNA samples or treated samples. Please do not bring samples containing live pathogens to the workshop.

Instrument and Software Training

7900HT Fast Real-Time PCR System

 

Need Additional Info?

Contact Al Agellon agellon@u.arizona.edu